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From 2011.igem.org

pET15b OmpA-GFP construct

 Pick 6 colonies from the plates.
 Inoculate 5ml LB Amp+.
 Culture PCR.
 Dip less than 1 ul bacteria culture (~3hr in 37 centigrade) into a PCR reaction mix with GFP primers.
 All of the clones have the GFP inserts
 Mini-prep the vectors.