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From 2011.igem.org

pET15b OmpA-GFP construct

 PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector.
 Digest with NdeI, BamHI (Takara) 5 hours.
 PCR OmpA 540bp fragment from BL21 genome.
 Digest with NcoI (NEB) 3 hours.
 -YX