File:20110709 MorePromoter combo 1.jpg

From 2011.igem.org

Gel of more transformants that should have esp + rsaA combo inserted behind Pxyl. Lane 1: ladder; lanes 2-4: combo from miniprep digest and gel extract insert; lanes 5-7: combo from PCR insert; lane 8: confirmation of insertion behind BBa_K081005; lane 9: standard combo from gel extraction. Insertion behind BBa_K081005 is cofirmed as successful, but none of the insertions behind Pxyl were successful. Also, the gel extract used as standard was more dilute than expected and did not show up on this gel.

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current16:14, 12 July 2011Thumbnail for version as of 16:14, 12 July 2011449×414 (9 KB)Aaring (Talk | contribs) (<html> Gel of more transformants that should have <i>esp</i> + <i>rsaA</i> combo inserted behind P<sub>xyl</sub>. Lane 1: ladder; lanes 2-4: combo from miniprep digest and gel extract insert; lanes 5-7: combo from PCR insert; lane 8: confirmation of inser)

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