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File:20110705 notCombo.jpg
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Digested miniprep samples of overnight cultures of transformation product that may contain esp and rsaA. Lane 1: esp standard; lanes 2,3: rsaA from gel extraction inserted into pSB1C3 containing esp from plates spread with 20μL of transformation cells; lanes 4,5: rsaA from gel extraction inserted into pSB1C3 containing esp from plates spread with 200μL of transformation cells; lanes 6,7: esp from gel extraction inserted into pSB1C3 containing rsaA from plates spread with 20μL of transformation cells; lanes 8,9: esp from gel extraction inserted into pSB1C3 containing rsaA from plates spread with 200μL of transformation cells; lane 10: ladder. Presence of original pieces and various bands that could be attributed to undigested or partially digested plasmid are apparent, but the combo is not visible. The gel is inconclusive, so follow-ups are being made.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 20:52, 5 July 2011 | | 838×694 (23 KB) | Aaring (Talk | contribs) | (<html>Digested miniprep samples of overnight cultures of transformation product that may contain <i>esp</i> and <i>rsaA</i>. Lane 1: <i>esp</i> standard; lanes 2,3: <i>rsaA</i> from gel extraction inserted into pSB1C3 containing <i>esp</i> from plates spr) |
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