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File:20110629 PromoterInserts Both.jpg
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Gel 3 of 3. PCR product of transformation colonies that should contain an insert of either Pxyl or PrsaA into pSB1C3 containing esp and rsaA C-terminal.
Lane 1: ladder; lanes 2,3: Pxyl insert PCR using plasmid primers VF2 and VR; lanes 4,5: PrsaA insert PCR using plasmid primers VF2 and VR; lanes 6,7: Pxyl insert PCR using promoter specific forward primer and VR; lanes 8,9: PrsaA insert PCR using promoter specific forward primer and VR. Lanes 8 and 9 suggest a successful insertion of PrsaA, however all of the bands are too small. This suggests to us that our esp + rsaA C-terminal may not contain esp at all, but rather only rsaA C-terminal.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:43, 30 June 2011 | | 1,066×790 (20 KB) | Aaring (Talk | contribs) | (Gel 3 of 3. PCR product of transformation colonies that should contain an insert of either P<html><sub>xyl</sub> or P<sub>rsaA</sub></html> into pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2,3: P<html><sub>xyl</sub> insert P) |
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