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File:20110623 esp+rsaA 135.jpg
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Gel 1 of 2.
Colony PCR of transformation products of insertions of esp from PCR into pSB1C3 containing rsaA C-terminal, insertions of rsaA C-terminal into pSB1C3 containing esp, and esp and rsaA C-terminal digests from pSB1C3 into pMR10 as a three piece ligation.
Lane 1: ladder; lanes 2-4: esp from PCR inserted into rsaA containing pSB1C3; lanes 5-7: rsaA from PCR inserted into esp containing pSB1C3; lanes 8-10: esp and rsaA digests from plasmid insertion into pMR10.
Lanes 2-4 show correct insertion length, but none of the other fragments are the correct length.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 23:00, 24 June 2011 | | 1,010×674 (16 KB) | Aaring (Talk | contribs) | (Gel 1 of 2. Colony PCR of transformation products of insertions of ''esp'' from PCR into pSB1C3 containing ''rsaA'' C-terminal, insertions of ''rsaA'' C-terminal into pSB1C3 containing ''esp'', and ''esp'' and ''rsaA'' C-terminal digests from pSB1C3 in) |
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