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File:20110621 espAndCombo.jpg
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Lanes 1-6: plasmid PCR of transformed cells that should carry the desired esp insert; lane 7: ladder; lanes 8-10: plasmid PCR of transformed cells that should be carrying esp + rsaA C-terminal insert. A few of the esp inserts succeeded, but as expected the three piece ligation failed again.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 21:02, 22 June 2011 | | 792×790 (16 KB) | Aaring (Talk | contribs) | (Lanes 1-6: plasmid PCR of transformed cells that should carry the desired ''esp'' insert; lane 7: ladder; lanes 8-10: plasmid PCR of transformed cells that should be carrying ''esp'' + ''rsaA'' C-terminal insert. A few of the ''esp'' inserts succeeded, b) |
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