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From 2011.igem.org

One possible idea for a very small protein diffusion is to make the Xis excisase protein diffuse through the nanotubes. The system can be sum up with the following scheme:

The problem of this design is the stoechasticity of the excision process. The process may occurs with a low probability wich will end up with a large discrepency in the excision time obtained, independantly of the diffusion of the molecule through the nanotubes.