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Thursday, July7
From 2011.igem.org
(Created page with "PCR recipe Date: 7/7 Control A Control B Rx1 A Rx1 B Rx2 A Rx2 B Rx3 A Rx3 B Rx4 A Rx4 B Rx5 A Rx5 B Polymerase (pfu Ultra) 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 10X b...") |
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Polymerase (pfu Ultra) 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul | Polymerase (pfu Ultra) 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul | ||
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10X buffer 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul | 10X buffer 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul | ||
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Forward primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul | Forward primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul | ||
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Reverse primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul | Reverse primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul | ||
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Plasmid 2ul 2ul 0.5ul 0.5ul 1ul 1ul 5ul 5ul 10ul 10ul 15ul 15ul | Plasmid 2ul 2ul 0.5ul 0.5ul 1ul 1ul 5ul 5ul 10ul 10ul 15ul 15ul | ||
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dNTPs 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul | dNTPs 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul | ||
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Nuclease free water 34.5ul 34.5ul 26ul 26ul 25.5ul 25.5ul 21.5ul 21.5ul 16.5ul 16.5ul 11.5ul 11.5ul | Nuclease free water 34.5ul 34.5ul 26ul 26ul 25.5ul 25.5ul 21.5ul 21.5ul 16.5ul 16.5ul 11.5ul 11.5ul | ||
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this it the recipe for the pcr reaction of mutagenesis we did on july 7 thursday. | this it the recipe for the pcr reaction of mutagenesis we did on july 7 thursday. | ||
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Cylcle temperature time | Cylcle temperature time | ||
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Initial warmup 95 degrees 2 mins | Initial warmup 95 degrees 2 mins | ||
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Denaturation 95 degrees 30 sec | Denaturation 95 degrees 30 sec | ||
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annealing 61.8 degrees 30 sec | annealing 61.8 degrees 30 sec | ||
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extension 72 degrees 5min | extension 72 degrees 5min | ||
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final extension 72 degrees 10 min | final extension 72 degrees 10 min | ||
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store 4 degrees infinitive | store 4 degrees infinitive | ||
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Bivor | Bivor | ||
Latest revision as of 01:26, 1 August 2011
PCR recipe Date: 7/7 Control A Control B Rx1 A Rx1 B Rx2 A Rx2 B Rx3 A Rx3 B Rx4 A Rx4 B Rx5 A Rx5 B Polymerase (pfu Ultra) 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul
10X buffer 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul 5ul
Forward primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul
Reverse primer (100ng/ul)1.25ul 1.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul 6.25ul
Plasmid 2ul 2ul 0.5ul 0.5ul 1ul 1ul 5ul 5ul 10ul 10ul 15ul 15ul
dNTPs 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul 1ul
Nuclease free water 34.5ul 34.5ul 26ul 26ul 25.5ul 25.5ul 21.5ul 21.5ul 16.5ul 16.5ul 11.5ul 11.5ul
this it the recipe for the pcr reaction of mutagenesis we did on july 7 thursday.
Cylcle temperature time
Initial warmup 95 degrees 2 mins
Denaturation 95 degrees 30 sec
annealing 61.8 degrees 30 sec
extension 72 degrees 5min
final extension 72 degrees 10 min
store 4 degrees infinitive
Bivor
