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<li><a class="head" href="#"> Introduction</a>
 
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<li><a href="/Team:Cambridge/TheTeam">Meet the Team</a></li>
 
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<li><a href="/Team:Cambridge/Photos">Photo Gallery</a></li>
 
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<li><a href="/Team:Cambridge/Videos">Videos</a></li>
 
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<li><a href="/Team:Cambridge/BioBricks">Parts submitted</a></li>
 
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</li>
 
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<li><a class="head" href="#">Project Firefly</a>
 
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<ul class="llmenu llmenugreen">
 
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<li><a href="/Team:Cambridge/Bioluminescence">Introduction</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Background_Firefly">Background</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Colour">Coloured outputs</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Luciferin_Regeneration">Luciferin recovery</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Firefly_Modelling">Modelling</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Firefly_Characterisation">Characterisation</a></li>
 
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<ul class="llmenu llmenublue">
 
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<li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">Introduction</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Background">Background</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/G28">The LuxBrick</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Codon_optimisation">Codon optimisation</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Modelling">Modelling</a></li>
 
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<li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Characterisation">Characterisation</a></li>
 
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<ul class="llmenu llmenured">
 
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<li><a href="/Team:Cambridge/Tools/Gibson">Gibthon</a></li>
 
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<li><a href="/Team:Cambridge/Tools/GenBank">BioBrick → GenBank</a></li>
 
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<li><a href="/Team:Cambridge/Tools/Ligate">Ligation Calculator</a></li>
 
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<li><a href="/Team:Cambridge/Tools/Eglometer">The E.glometer</a></li>
 
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<li><a class="head" href="#">Gibson Assembly</a>
 
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<ul class="llmenu llmenublue">
 
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<li><a href="/Team:Cambridge/Gibson/Introduction">Introduction</a></li>
 
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<li><a href="/Team:Cambridge/Gibson/Mechanism">How it works</a></li>
 
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<li><a href="/Team:Cambridge/Gibson/Protocol">Protocol</a></li>
 
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<li><a href="/Team:Cambridge/Gibson/RFC">RFC</a></li>
 
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<li><a class="head" href="#">Considerations</a>
 
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<ul class="llmenu llmenublue">
 
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<li><a href="/Team:Cambridge/Human_Practices">Human Practices</a></li>
 
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<li><a href="/Team:Cambridge/Safety">Safety</a></li>
 
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</ul>
 
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<li><a class="head" href="#">Future applications</a>
 
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<ul class="llmenu llmenured">
 
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<li><a href="/Team:Cambridge/Tools/microMeasure">Biosensors</a></li>
 
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<li><a href="/Team:Cambridge/Tools/Lighting">Lighting</a></li>
 
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</ul>
 
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</li>
 
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<li><a class="head" href="/Team:Cambridge/Notebook/Week1">Notebook</a>
 
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<ul class="llmenu llmenuyellow">
 
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<li><a href="/Team:Cambridge/Notebook/Summary">Summary</a></li>
 
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<li><a href="/Team:Cambridge/Notebook/Week1">Week 1</a></li>
 
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<li><a href="/Team:Cambridge/Notebook/Week2">Week 2</a></li>
 
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<li><a href="/Team:Cambridge/Notebook/Week3">Week 3</a></li>
 
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<li><a href="/Team:Cambridge/Notebook/Week4">Week 4</a></li>
 
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<li><a href="/Team:Cambridge/Notebook/Week5">Week 5</a></li>
 
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<strong class="selflink">Making long term stocks</strong> | <a href="/Team:Cambridge/Protocols/Comp" title="Team:Cambridge/Protocols/Comp">Transformation</a>  | <a href="http://microscopy.berkeley.edu/Resources/instruction/buffers.html" class="external text" title="http://microscopy.berkeley.edu/Resources/instruction/buffers.html" rel="nofollow">Buffer recipes</a> | <a href="/Team:Cambridge/Protocols/ColonyPCR" title="Team:Cambridge/Protocols/ColonyPCR">Colony PCR</a>
 
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<a name="Making_long_term_stocks_in_glycerol"></a><h1><span class="editsection">[<a href="/wiki/index.php?title=Team:Cambridge/Protocols/Long&amp;action=edit&amp;section=1" title="Edit section: Making long term stocks in glycerol">edit</a>]</span> <span class="mw-headline">Making long term stocks in glycerol</span></h1>
 
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<p>(From <a href="http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria" class="external free" title="http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria" rel="nofollow">http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria</a>)
 
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<a name="Materials"></a><h2><span class="editsection">[<a href="/wiki/index.php?title=Team:Cambridge/Protocols/Long&amp;action=edit&amp;section=2" title="Edit section: Materials">edit</a>]</span> <span class="mw-headline">Materials</span></h2>
 
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<ul><li>40% glycerol solution
 
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</li><li>Cryogenic vials
 
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<ul><li>Add 1 ml of 40% glycerol in H<sub>2</sub>O to a cryogenic vial.
 
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</li><li>Add 1 ml sample from the culture of bacteria to be stored.
 
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</li><li>Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
 
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<ul><li>Alternatively, pipet to mix.
 
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</li><li>Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
 
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</li><li>On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
 
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</li><li>Store in a freezer box in a -80C freezer.  Remember to record where the vial is stored for fast retrieval later.
 
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<ul><li>While it is possible to make a long term stock from cells in stationary phase, ideally your culture should be in logarithmic growth phase.
 
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