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Team:uOttawa/Project
From 2011.igem.org
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Overall project
One of the major goals of synthetic biology is to apply engineering principles analogous to traditional engineering disciplines to genetic engineering. A way in which this is achieved is through the use of biobricks that allow for more rapid engineering of synthetic gene networks. These biobricks are often assembled and tested in a bacterial chassis however there remains a shortage of these biobricks optimized to work in eukaryotes. To this end, we are building several biobricks optimized to work in the model organism S. cerevisiae - a simple eukaryote as well as building a novel yeast chassis that will allow for the robust characterization of these biobricks. In addition, we are developing a new protocol that will greatly improve upon the existing assembly protocol for biobricks and allow for rapid integration into S. cerevisiae.
