Team:uOttawa

From 2011.igem.org

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<p>Brewer's yeast has become a model organism for the study of eukaryotes due to its homology with mammalian organisms and its ease of genetic manipulation. For this reason, we believe it is important to develop tools that improve our ability to use yeast as a standard BioBrick organism. To this end, we aim to submit several BioBricks optimized to work in the simple eukaryote ''S. cerevisiae'' as well as build a novel yeast chassis that is capable of the robust characterization of these Biobricks. In addition, we are developing a new assembly protocol that will greatly improve upon existing BioBrick assembly protocols and allow for rapid integration into ''S. cerevisiae''.</p>
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<p>The budding yeast Saccharomyces cerevisea has emerged as an important micro-organisms on several fronts. Not only is this yeast important model organism due to its homology to higher eukaryotes, but it sees wide commercial application as well. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in S. cerevisiae. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have submitted several BioBricks optimized for use in S. cerevisiae. We have also succeeded in developing a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols, not just for yeast but for the who BioBrick community.</p>
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Revision as of 02:52, 29 September 2011


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The budding yeast Saccharomyces cerevisea has emerged as an important micro-organisms on several fronts. Not only is this yeast important model organism due to its homology to higher eukaryotes, but it sees wide commercial application as well. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in S. cerevisiae. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have submitted several BioBricks optimized for use in S. cerevisiae. We have also succeeded in developing a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols, not just for yeast but for the who BioBrick community.

Team

This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.

Project

Characterization and standardizing of biological parts is a continuing problem for synthetic biologists. This year we decided to focus on developing a more efficient assembly method as well as optimizing characterization of BioBricks in S. cerevisiae.

Results

We were able to successfully create a new assembly method as well as characterize a number of transcription factors.

Parts Submitted

We submitted 7 new biobricks to the registry this year.

Human Practices

In order for the field of Synthetic Biology to grow, more youth outreach is needed. The uOttawa team created a game to teach simple synbio theory and has arranged high school tutorials for the fall. Also check out our essay on the public view on Synthetic Biology and ways of improving it.

Sponsors

The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.