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Team:ZJU-China/Sugar-Design.html
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<p><partinfo><strong>BBa_K561004 Sequence And | <p><partinfo><strong>BBa_K561004 Sequence And | ||
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<p>In the Sugarfilm expression system, <a | <p>In the Sugarfilm expression system, <a | ||
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Revision as of 16:25, 19 October 2011
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Biofilm
Rainbofilm
Sugarfilm
Parts
Achievements
Tools
Design
In the Sugarfilm expression system, BBa_K561004 is designed for bottom layer expression, conducting the first step of cellulose degradation. It is based on Rainbofilm bottom layer BioBrick BBa_K561002 , with substitution of mRFP (BBa_E1010 ) by Cex and CenA. The sequence is under regulation of anaerobic promoter fdhF, and the TetR expression would repress upper-layer device (Ptet regulated) in anaerobic condition, i.e. in the bottom-layer condition.
BBa_K561005 is designed for medium layer expression, conducting the conversion of cellubiose into glucose. It is based on Rainbofilm bottom layer BioBrick BBa_K561000 , with substitution of eYFP (BBa_E0030 ) by BglX. The sequence is under regulation of micro-aerobic promoter vgb, and the TetR expression would repress upper-layer device (Ptet regulated) in micro-anaerobic condition, which mostly exists in the middle part of the biofilm.
Coding sequence with regulation of Ptet would be suitable as the upper-layer device (such as BBa_I13602 used in Rainbofilm system). Expression of the device would be repressed either in the bottom or medium layer, and would do its job faithfully in the upper-layer region.
