Team:ZJU-China/Safety.html

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<h4>Overview</h4>
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<div class="pane" style="height: auto"><a
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href="https://2011.igem.org/Team:ZJU-China/Humanpractice.html">Overview</a></div>
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<h4 class="current">Safety</h4>
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<div class="pane" style="display: block; height: auto"><a
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href="https://2011.igem.org/Team:ZJU-China/Safety.html">Safety<br />
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</a></div>
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<h4>Originality</h4>
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App<br />
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</a> <a href="https://2011.igem.org/Team:ZJU-China/Novel.html">Novel<br />
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</a></div>
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</a></div>
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href="https://2011.igem.org/Team:ZJU-China/SBC.html">SBC<br />
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<h1>Safety</h1>
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<div class="block" id="safety1">
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<h1>Researcher safety</h1>
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<hr />
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<p>Our researchers mainly face regular bio-safety challenges in a
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molecular microbiology lab, such as risks staining DNA agarose gel with
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EB, extracting RNA from organisms with TRIZOL, and manipulating with
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DEPC treated water, etc. Any researchers conducting such experiment<img
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src="https://static.igem.org/mediawiki/2011/4/42/ZJUsafety2.jpg" width="300"
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style="float: right;" /> are instructed about the right method both by
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printed protocols and BIG BROTHER in the lab (XD). After the training,
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we must pass an on-line test about the lab safety, standard experiment
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procedure and lab waste treatment. During any experiments, necessary
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individual protections such as gloves and white robes are always
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required. Also, the public safety of the lab is guaranteed by the strict
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registering protocols on the operation of any apparatus. In order to
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make sure all the requirements above were followed well by the
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researchers and to get prepared for any emergency, our lab (also is the
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open bio-lab of the college) set up 24hr*7 camera system in the room.</p>
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<div class="block" id="safety1">
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<h1>Public safety</h1>
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<hr />
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<p>First we picked DH5α and JM109 as the two strains to manipulate
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in our research. These are two non-pathogenic E. coli strain and do not
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pose problems to the public health. The bio-parts we utilized are all
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security ensured, coding for florescence protein, cellulose degradation
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enzymes and metal ion binding proteins. Although bio-film formation is
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closely related to pathogenicity, our experiment enhances bio-film
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formation only by changing the external environment factors instead of
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modifying the inherited nature of the bacteria. This is an important
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measure to assure that the bacteria do not acquire previously
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non-existing pathogenic capacity.</p>
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</div>
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<div class="block" id="safety1">
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<h1>Environment safety</h1>
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<hr />
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<p>The experiments conducted in our lab are with intensive concern
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of environmental impact. No media containing engineered germs go into
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the sewer before sterilization. Besides, all the utensil contacted with
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the engineered bacteria must first undergo sterilization then be washed.
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During our research we have used antibiotic resistant plasmids,
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including chloramphenicol, kanamycin, ampicillin. Though risks of the
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practices exist, we believe that it's possible for us to use them safely
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with the mature protocols and techniques. For the toxic and hazardous
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chemicals, they are collected and treated properly. The acid and basic
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chemicals are neutralized and pour into the swer.</p>
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<div class="block" id="safety1">
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<h1>Biobrick safety</h1>
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<hr />
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<p><img src="https://static.igem.org/mediawiki/2011/4/46/Zjusafety1.jpg"
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width="220" style="float: left;">Our biobricks includes two new
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promoters responded to oxygen concentration, hence the regulation of the
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devices would not involve potentially bio-hazard chemicals. Also as
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partially described above, our parts express proteins aiming at binding
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of metal ions, degradation of cellulose, and the localization by
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fluorescent proteins. All of these products are not bio-hazard, and the
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expression is under fine control.</p>
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<div class="block" id="safety1">
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<h1>Biosafety provisions</h1>
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<p>Our experiment is designed and carried out following the
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standards of national biosafety office, especially the <a
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href="http://www.biosafety.gov.cn/gjzcfg/flfg/200401/t20040115_88044.htm"
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target="_blank">《Safety Administration Regulation on Genetic
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Engineering》</a> (Only the chinese version is available online),as well as <a
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href="http://www.zjubiolab.zju.edu.cn/page/news.php?action=introlist&news_catalogId=33"
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target="_blank">the laboratory regulations</a> of our university, the
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college of life sciences, and the biology lab center.</p>
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<p><a name="jumptop" id="bb">&nbsp; Biobrick Group&nbsp;</a>|<a
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Revision as of 17:27, 19 October 2011

  1. REDIRECT Safety
Retrieved from "http://2011.igem.org/Team:ZJU-China/Safety.html"