Team:Yale/Notebook/Week6

From 2011.igem.org

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<li>Survival assays:
<li>Survival assays:
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<ul><li>Completed survival assays on induced and uninduced MBP-TmAFP, eGFP-TmAFP, eGFP-TEV-TmAFP samples to determine greatest freeze-thaw viability (see figure:)</li>
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<ul><li>Completed survival assays on induced and uninduced MBP-TmAFP, eGFP-TmAFP, eGFP-TEV-TmAFP samples to determine greatest freeze-thaw viability (see assays page)</li>
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<li>More detailed descriptions in physical lab notebook</li>
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Latest revision as of 01:35, 29 September 2011

iGEM Yale

Week 6: June 19-June 26

  • Cloning struggles:
    • Multiple attempts to PCR amplify ZeAFP with Taq and Phusion polymerase failed
    • Eventually succeed with Taq, digest with XbaI and SpeI
    • Ligate with T4 DNA ligase
  • Survival assays:
    • Completed survival assays on induced and uninduced MBP-TmAFP, eGFP-TmAFP, eGFP-TEV-TmAFP samples to determine greatest freeze-thaw viability (see assays page)
    • More detailed descriptions in physical lab notebook

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