"
Team:Yale/Notebook/Week4
From 2011.igem.org
Notebook: Week 4
Monday
- Made 1 L LB, completed 1/40 dilution of ZeAFP (added Amp), shook for 3 hrs, checked OD until .78 OD reached
- Divided 1 L into 6 separate 100 mL solutions, half with IPTG, induced for 5 hours
- Centrifuged 4 100 mL solutions, saved supernatant/pellet for 2; for the other 2, we completed the following protocol:
1) Washed with 50 mL water
2) Resuspend in 100 mL ice-cold water
3)
- Plated 6 samples of the remaining 2 100 mL solutions, using 10 and 100 microliters on IPTG, LB, Amp plates and non-IPTG, LB, Amp plates and stored 1 of each concentration in 0 degrees, 4 degrees, and room temperature
Tuesday
- Finished and presented project defense presentation to Sackler Institute and our advisers.
Wednesday
Plan:
Accomplished:
Thursday
Plan:
Accomplished:
Friday
Plan:
Accomplished:
