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Team:Yale/Notebook/Week4
From 2011.igem.org
(Created page with "== Notebook: Week 4 == ''Monday'' Plan: Accomplished: ''Tuesday'' Plan: Accomplished: ''Wednesday'' Plan: Accomplished: ''Thursday'' Plan: Accomplished: ''Friday...") |
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''Monday'' | ''Monday'' | ||
| - | + | - Made 1 L LB, completed 1/40 dilution of ZeAFP (added Amp), shook for 3 hrs, checked OD until .78 OD reached | |
| - | + | - Divided 1 L into 6 separate 100 mL solutions, half with IPTG, induced for 5 hours | |
| - | + | - Centrifuged 4 100 mL solutions, saved supernatant/pellet for 2; for the other 2, we completed the following protocol: | |
| - | + | 1) Washed with 50 mL water | |
| + | 2) Resuspend in 100 mL ice-cold water | ||
| + | 3) | ||
| + | - Plated 6 samples of the remaining 2 100 mL solutions, using 10 and 100 microliters on IPTG, LB, Amp plates and non-IPTG, LB, Amp plates and stored 1 of each concentration in 0 degrees, 4 degrees, and room temperature | ||
''Tuesday'' | ''Tuesday'' | ||
| - | + | - Finished and presented project defense presentation to Sackler Institute and our advisers. | |
| - | + | ||
| - | + | ||
| - | + | ||
Revision as of 20:38, 7 June 2011
Notebook: Week 4
Monday - Made 1 L LB, completed 1/40 dilution of ZeAFP (added Amp), shook for 3 hrs, checked OD until .78 OD reached - Divided 1 L into 6 separate 100 mL solutions, half with IPTG, induced for 5 hours - Centrifuged 4 100 mL solutions, saved supernatant/pellet for 2; for the other 2, we completed the following protocol: 1) Washed with 50 mL water 2) Resuspend in 100 mL ice-cold water 3) - Plated 6 samples of the remaining 2 100 mL solutions, using 10 and 100 microliters on IPTG, LB, Amp plates and non-IPTG, LB, Amp plates and stored 1 of each concentration in 0 degrees, 4 degrees, and room temperature
Tuesday
- Finished and presented project defense presentation to Sackler Institute and our advisers.
Wednesday
Plan:
Accomplished:
Thursday
Plan:
Accomplished:
Friday
Plan:
Accomplished:
