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From 2011.igem.org

Notebook: Week 3

Monday - Food

Tuesday - Darren arrives

- Discussion of R. inquisitor

Wednesday - Tokyo Tech iGEM Plasmid (TmAFP) and Canada, Davies Lab Plasmid (ZeAFP) arrived, streaked 1 plate for each plasmid

- Modelling/homology study for RiAFP

- Afternoon meeting with Prof. Farren Isaacs about project and gene we're looking to synthesize

- Emailed companies for sponsorship for gene synthesis

- Emailed other iGEM teams for suggestion

Thursday - Found protocol for Origami cell lines at Xiong lab

- Designed gene on IDT website

Friday - Asking about TEV, GFP, His

- Ordered gene

- Contacted Israeli lab about Plasmids

Saturday - Miniprep, Nanodrop

- Cultured 2 colonies in 100 mL flasks

- Warren worked on primer design

Sunday - 1/40 dilution in 1 liter

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