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Team:Yale/Notebook/Week3
From 2011.igem.org
Notebook: Week 3
Monday - Food
Tuesday - Darren arrives - Discussion of R. inquisitor
Wednesday - Tokyo Tech iGEM Plasmid (TmAFP) and Canada, Davies Lab Plasmid (ZeAFP) arrived, streaked 1 plate for each plasmid - Modelling/homology study for RiAFP - Afternoon meeting with Prof. Farren Isaacs about project and gene we're looking to synthesize - Emailed companies for sponsorship for gene synthesis - Emailed other iGEM teams for suggestion
Thursday - Found protocol for Origami cell lines at Xiong lab - Designed gene on IDT website
Friday - Asking about TEV, GFP, His - Ordered gene - Contacted Israeli lab about Plasmids
Saturday - Miniprep, Nanodrop - Cultured 2 colonies in 100 mL flasks - Warren worked on primer design
Sunday - 1/40 dilution in 1 liter
