== Notebook: Week 3 ==
''Monday''
- Food
''Tuesday''
- Darren arrives
- Discussion of R. inquisitor
''Wednesday''
- Tokyo Tech iGEM Plasmid (TmAFP) and Canada, Davies Lab Plasmid (ZeAFP) arrived, streaked 1 plate for each plasmid
- Modelling/homology study for RiAFP
- Afternoon meeting with Prof. Farren Isaacs about project and gene we're looking to synthesize
- Emailed companies for sponsorship for gene synthesis
- Emailed other iGEM teams for suggestion
''Thursday''
- Found protocol for Origami cell lines at Xiong lab
- Designed gene on IDT website
''Friday''
- Asking about TEV, GFP, His
- Ordered gene
- Contacted Israeli lab about Plasmids
''Saturday''
- Miniprep, Nanodrop
- Cultured 2 colonies in 100 mL flasks
- Warren worked on primer design
''Sunday''
- 1/40 dilution in 1 liter
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