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Team:Yale/Notebook/Week2
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''Monday'' | ''Monday'' | ||
| - | + | - Meeting scheduled in the Med School TAC building with Martin and Girish tomorrow at 2:30pm about using their plasmids with the anti-freeze glycoprotein that they isolated. | |
| + | - Meeting scheduled for Friday with Farren at 2:30pm in West Campus, building B-31, Room 209. | ||
| - | + | - Made TB buffer (DH5-alpha competent cell buffer). 5mLs of 1M HEPES buffer (final concentration 10mM), 75mL of 0.1M CaCL2*2H2O (final concentration 15mM), 9.32g KCl (final concentration 250mM) was added to a beaker. Volume was brought to 300mL. pH was brought to 6.7 with 6.25M KOH. 5.44g of MnCL2*4H2O (final concentration 55mM) was added, and the volume was brought to 500mL. The contents of the beaker were sterile filtered into a sterile bottle purchased from the basement store. | |
| + | - Made 500mL LB buffer, which was distributed in 2x250mL Erlenmeyer flasks and 2x500mL Erlenmeyer flasks. No antibiotic was added. | ||
''Tuesday'' | ''Tuesday'' | ||
Revision as of 19:16, 23 May 2011
Notebook: Week 2
Monday
- Meeting scheduled in the Med School TAC building with Martin and Girish tomorrow at 2:30pm about using their plasmids with the anti-freeze glycoprotein that they isolated. - Meeting scheduled for Friday with Farren at 2:30pm in West Campus, building B-31, Room 209.
- Made TB buffer (DH5-alpha competent cell buffer). 5mLs of 1M HEPES buffer (final concentration 10mM), 75mL of 0.1M CaCL2*2H2O (final concentration 15mM), 9.32g KCl (final concentration 250mM) was added to a beaker. Volume was brought to 300mL. pH was brought to 6.7 with 6.25M KOH. 5.44g of MnCL2*4H2O (final concentration 55mM) was added, and the volume was brought to 500mL. The contents of the beaker were sterile filtered into a sterile bottle purchased from the basement store.
- Made 500mL LB buffer, which was distributed in 2x250mL Erlenmeyer flasks and 2x500mL Erlenmeyer flasks. No antibiotic was added.
Tuesday
Plan:
Accomplished:
Wednesday
Plan:
Accomplished:
Thursday
Plan:
Accomplished:
Friday
Plan:
Accomplished:
