Team:Yale/Notebook/Week13

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<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week10">Week 10</a></li>
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week10">Week 10</a></li>
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week11">Week 11</a></li>
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week11">Week 11</a></li>
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<li><a id="page" href="https://2011.igem.org/Team:Yale/Notebook/Week12">Week 12</a></li>
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<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week12">Week 12</a></li>
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<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week13">Week 13</a></li>
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<li><a id="page" href="https://2011.igem.org/Team:Yale/Notebook/Week13">Week 13</a></li>
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week14">Week 14</a></li>
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week14">Week 14</a></li>
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week15">Week 15</a></li>
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week15">Week 15</a></li>
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<li>Grew up another cell culture for protein prep</li>
<li>Grew up another cell culture for protein prep</li>
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Latest revision as of 05:45, 28 September 2011

iGEM Yale

Week 13: August 7-14, 2011

  • Monday:
    • Spin-concentrated all protein samples to 5-10 mg/mL
    • Resuspended and sonicated cell pellets
    • Used mosquito to set up Wizard and PEG crystal screen for eGFP-RiAFP and RiAFP
    Tuesday:
    • Meeting with Prof. Isaacs about specific MAGE oligo designs and iGEM updates
    • Obtained plates of EcNR2 strain and Spec/Kan antibiotic plasmids
  • Wednesday:
    • Innoculated ECNr2 strains and prepared glycerol stocks
    • Designed primers for cross-over PCR to integrate RiAFP and T7 cassette into EcNR2 genome pre-MAGE
  • Thursday:
    • Found successful fuzzy-ball and shower crystals! (see image:)
    • Ran Lambda DE3 kit on EcNR2 strain as alternative means of integrating T7 polymerase into genome
    • Set up PCR amplification of pre-crossover fragments of Spectinomycin, Kanamycin cassettes and RiAFP, eGFP-RiAFP, and T7 polymerase, with ends that correspond to high-recombination frequency sites
  • Friday:
    • Grew up another cell culture for protein prep

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