Team:Wisconsin-Madison/projectoverview

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<a href="https://2011.igem.org/Team:Wisconsin-Madison/umad">Main</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison">Main</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ca">Contributions & Attributions</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamethanol">Ethanol Sensor</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamalkane">Alkane Sensor</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teambmc">Microcompartment</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">Directed Evolution</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/parts">Parts</a>
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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview" onmouseover="mopen('m3')" onmouseout="mclosetime()">Team</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview">Overview</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teammembers">Members</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teammembers">Members</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamfaculty">Faculty</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamadvisors">Advisors</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamsponsors">Sponsors</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamsponsors">Sponsors</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookprotocols">Protocols</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookcalender">Calender</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookexperiements">Experiment</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/references">References</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/presentations">Presentations</a>
 
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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/safety" onmouseover="mopen('m6')" onmouseout="mclosetime()">Safety</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/humanpractice">Human Practice</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Project</a> >>  
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Project</a> >>  
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>,  
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>,  
<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>,  
<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>,  
<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>,  
<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>,  
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">BMC</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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Mary Sagstetter:<p>
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I am a senior at University of Wisconsin-Madison majoring in Microbiology and Life Science Communication. My research interest is in applying microbiology to public health related fields such as monitoring antibiotic resistance in a waste water treatment plant. I have also written science articles for the college newspaper that pertain to student health issues and research around the campus. This is my second year on the iGEM team. Fun Fact: The picture was taken at the hockey rink inside Camp Randall.
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Eric Walters: <p>
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Project Overview
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I am a senior at University of Wisconsin-Madison majoring in Microbiology and Life Science Communication. My research interest is in applying microbiology to public health related fields such as monitoring antibiotic resistance in a waste water treatment plant. I have also written science articles for the college newspaper that pertain to student health issues and research around the campus. This is my second year on the iGEM team. Fun Fact: The picture was taken at the hockey rink inside Camp Randall.
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The UW-Madison iGEM team is working on a biofuel <a href="https://2011.igem.org/Team:Wisconsin-Madison/biosensor">biosensor</a> project for 2011. This project ultimately breaks down into several smaller projects, each described in greater detail in their respective sections: an<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol"> ethanol sensor</a>, an <a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">alkane sensor</a>, and a <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">directed evolution project</a> for each sensor. We originally intended to use <a href=" https://2011.igem.org/Team:Wisconsin-Madison/bmc">bacterial microcompartments</a> (BMCs) to improve the efficacy of our sensors, however, due to time constraints the project was dropped.
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John De Friel: <p>
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Our biosensing systems are designed to produce a red fluorescent protein (RFP) in the presence of whatever they are designed to sense; in our case, either ethanol (EtOH) or n-alkanes. These two compounds are frequently used for biofuel.  Currently, common laboratory chromatography techniques are used to characterize biofuel production commercially. By using biosensors, we hope to provide an accurate, cheaper, and less time-consuming method of assessing <a href="https://2011.igem.org/Team:Wisconsin-Madison/biofuel">biofuel</a> production.  
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John De Friel is a senior in the department of Chemical and Biological Engineering at UW-Madison focusing on computational and experimental approaches to systems and synthetic biology. In his computational work, he develops and analyzes genome-scale metabolic models of bacteria. These models are used for determining genetic engineering strategies of bacteria to increase their production of bio-fuels or the degradation of environmental contaminants. His experimental work has focused on using synthetic biology to develop bio-sensors for petroleum products and working in a genomics laboratory to sequence and analyze the genome of Pseudomonas fluorescens L5.1-96.
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We found the idea of directed evolution very promising for improving our biosensors to increase commercial applicability, and wanted to do so in a way that would utilize the parts registry. We used <i>sacB</i> (BBa_K322921), a kanamycin selection, and a red fluorescent protein to produce a device that can be useful for future teams to improve their own one- or two-component sensor systems. As we worked with mutant libraries of BioBrick parts, we considered how directed evolution could fit into the registry. You can see our considerations in the <a href="https://2011.igem.org/Team:Wisconsin-Madison/humanpractice">human practice section</a>.
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Kenneth O. Xu <p>
 
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Kenny is a senior at the University of Wisconsin-Madison studying biomedical engineering. He enjoys listening to dubstep and making esports commentary in his free time.
 
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Ian Linsmeier <p>
 
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Ian Linsmeier is a junior majoring in Biomedical Engineering at the University of Wisconsin Madison. His research interests include tissue engineering and neurally controlled robotic limb prostheses. Recently, he just finished a pneumatic esophageal analog that was used to test and improve a developing medical device intended to replace the pulmonary artery catheter.
 
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Patrick Cassidy <p>
 
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Patrick Cassidy a senior at UW-Madison in the Biomedical Engineering department.  Unsure of his interests, he has worked in several labs; studying the biomechanics of ACL injuries, modeling the chemical interactions of transition metals and hydroxamic acids, and now using synthetic biology to produce bio-sensors. Recently, he has discovered an interest in the chemistry of polymeric materials, and helped design a protective gel for thermal ablation procedures, which is currently being pursued for a patent by WARF. Other interests are science fiction, heavy guitars, and the 80’s.
 
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Learn more about <a href="https://2011.igem.org/Team:Wisconsin-Madison/biosensor">biosensors</a>.
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<font size="1"><i>About the image: Our current system uses a two plasmid system that requires both arabinose and the desired analyte to produce RFP. Using an arabinose inducible promoter ultimately allows better control of the transcription of RFP.</i></font>
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Yaming Jiang <p>
 
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Yaming Jiang is a junior student in Chemical and biological engineering in University of Wisconsin Madison. With equal interests in biology and chemistry, she studied on human corneal cell’s morphology and growth orientation in respond to surface morphology and chemistry after freshman year to help improve corneal implant. Later, she worked on self-assembly bi-block copolymers and molecular transfer printing process, which has a promising future application in manufacturing computer chip with even smaller feature. She also did tutoring at College of Engineering undergraduate learning center. She likes reading fictions and swimming, and is especially happy about having Lake Mendota by the campus.
 
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Xiong Xiong <p>
 
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Xiong Xiong a senior at UW-Madison in the Chemical and Biological Engineering department. She used to work and study with systems biology experts in WID, focusing on the exploration of vesicular stomatitis  virus (VSV) and other RNA viruses. In the past year, she researched the effects of media nutrients on growths of VSV and host cells (Baby hamster cells, analogous to cancer cells). The essential efforts are devoted to model a “Best” virus to kill cancer cells without destroying healthy body cells. Now she is going in the design of a hydrocarbon biosensor and the application of bacterial microcompartments using synthetic biology. Her interests include travelling, jogging, cooking and watching movies.
 
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Latest revision as of 03:08, 29 September 2011









Project >> Overview, Ethanol Sensor, Alkane Sensor, Microcompartment

Project Overview

The UW-Madison iGEM team is working on a biofuel biosensor project for 2011. This project ultimately breaks down into several smaller projects, each described in greater detail in their respective sections: an ethanol sensor, an alkane sensor, and a directed evolution project for each sensor. We originally intended to use bacterial microcompartments (BMCs) to improve the efficacy of our sensors, however, due to time constraints the project was dropped.

Our biosensing systems are designed to produce a red fluorescent protein (RFP) in the presence of whatever they are designed to sense; in our case, either ethanol (EtOH) or n-alkanes. These two compounds are frequently used for biofuel. Currently, common laboratory chromatography techniques are used to characterize biofuel production commercially. By using biosensors, we hope to provide an accurate, cheaper, and less time-consuming method of assessing biofuel production.

We found the idea of directed evolution very promising for improving our biosensors to increase commercial applicability, and wanted to do so in a way that would utilize the parts registry. We used sacB (BBa_K322921), a kanamycin selection, and a red fluorescent protein to produce a device that can be useful for future teams to improve their own one- or two-component sensor systems. As we worked with mutant libraries of BioBrick parts, we considered how directed evolution could fit into the registry. You can see our considerations in the human practice section.


Learn more about biosensors.

About the image: Our current system uses a two plasmid system that requires both arabinose and the desired analyte to produce RFP. Using an arabinose inducible promoter ultimately allows better control of the transcription of RFP.



Retrieved from "http://2011.igem.org/Team:Wisconsin-Madison/projectoverview"