From 2011.igem.org
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| - | =Introduction=
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| - | We have developed a model of restricting gene expression to the RNA level through the sequencing and testing of self-excising introns. Using this model, we are able to predict the level at which specific sequences are suppressed and incorporate them into future project designs that may require this level of control. The Ribozyme Project aims to establish a novel way to make fusion proteins, therefore, it is important to ensure that the intron components will effectively remove an interrupting sequence and effectively re-ligate together, ensuring a functional protein product. Consequently, an easily detectable protein product must be interrupted with a sequence that is definite to either stop translation or alter the protein enough to render it ineffective. In our design, GFP is interrupted with a stop codon-containing sequence, which is flanked by the two intron parts. The parts submitted for this design were sequenced, digested and subsequently ligated into PSB1C3.
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Latest revision as of 16:52, 23 September 2011
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