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Team:Washington/Protocols/Digestion
From 2011.igem.org
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(→General Digestion Protocol) |
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10 uL DNA | 10 uL DNA | ||
| - | 5uL buffer ( | + | 5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers) |
| - | 0.5 uL BSA | + | 0.5 uL BSA (if required. See above) |
1uL enzyme 1 | 1uL enzyme 1 | ||
| - | |||
| - | |||
water to 50 uL (32.5 uL, add first) | water to 50 uL (32.5 uL, add first) | ||
| + | |||
| + | If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications. | ||
Latest revision as of 02:39, 16 September 2011
General Digestion Protocol
10 uL DNA
5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
0.5 uL BSA (if required. See above)
1uL enzyme 1
water to 50 uL (32.5 uL, add first)
If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
