Team:Washington/Protocols/Cyto.

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=Cytometry Protocol=
=Cytometry Protocol=

Latest revision as of 23:53, 28 September 2011


Cytometry Protocol

Colonies from plates:

  1. Stab cells from isolated colony on plate and suspend them in 50 uL PBS buffer.
  2. Dilute PBS culture by adding 1 uL to 100 uL fresh PBS buffer.
  3. Measure fluorescence levels on cytometer.


Cells from liquid culture:

  1. Grow cells to mid-exponential phase (generally, OD 0.1 to 0.2).
  2. Dilute cells 1:20 in PBS buffer supplemented with chloramphenicol or kanamycin (choose an antibiotic the cells aren't resistant to), and store on ice.
  3. Measure fluorescence levels on cytometer.
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