Team:Washington/Protocols

From 2011.igem.org

(Difference between revisions)
 
(78 intermediate revisions not shown)
Line 1: Line 1:
{{Template:Team:Washington/Templates/Top}}
{{Template:Team:Washington/Templates/Top}}
 +
__NOTOC__
-
INSERT INFO HERE... LOOK AT OTHER PAGES FOR EXAMPLES OF FORMATING AND INSERTING PICTURES.  CLICK THE EDIT BUTTON ON THE UPPER LEFT SIDE OF THE PAGE AFTER YOU HAVE SIGNED IN.
+
<center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br>
-
[https://2011.igem.org/Team:Washington/Alkanes/Protocols/Example_1 Example 1]
+
=General Protocols=
-
[https://2011.igem.org/Team:Washington/Alkanes/Protocols/Example_2 Example 2]
+
[https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/PCR  General PCR Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Digestion  General Digestion Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Ligation  General Ligation Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Transformation  General Transformation Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Colony Colony PCR Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Competent  Competent Cell Prep Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis]
 +
 
 +
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/gene_assembly Gene Assembly With Oligos]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/sequencing Sequencing]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/CompDesign  Computational Protein Design]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Glycerol_Stocks  Glycerol Stocks]
 +
 
 +
 
 +
=Make It:  Diesel Production Protocols=
 +
 
 +
[https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction]
 +
 
 +
[https://2011.igem.org/Team:Washington/alkanebiosynthesis_cloning Alkane Biosynthesis cloning]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team]
 +
 
 +
 
 +
=Break It:  Gluten Destruction Protocols=
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Whole Cell Lysate Assay]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay]
 +
 
 +
 
 +
=Make It: iGEM Toolkits=
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Elect. Electroporation (Transformation)]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Gib_Rxn Gibson Cloning/Assembly]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/High_PCR High-Yield PCR (Full-Gene Assembly)]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/pGA. pGA Vector Assay]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol]
 +
 
 +
[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]
 +
 
 +
 
 +
 
 +
=Wiki Design=
 +
[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)]

Latest revision as of 02:10, 29 September 2011


Protocols


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagenesis

Overview of the Kunkel Mutagenesis process

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks


Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Alkane Biosynthesis cloning

Cell Lysate Assay by Decarbonylase Redesign Team


Break It: Gluten Destruction Protocols

Whole Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification

Enzyme Assay


Make It: iGEM Toolkits

Cytometry Protocol

Electroporation (Transformation)

Gibson Cloning/Assembly

Gibson Purification

High-Yield PCR (Full-Gene Assembly)

Isolation of Plasmid DNA (miniprep)

Induction Studies of Proteins Fusions (mam-sfGFP)

pGA Vector Assay

PBS Stock Protocol

Preparation of Overnight Cultures


Wiki Design

Wiki Design Tools (Wiki Markup, WikiDust, etc.)