Team:Washington/Protocols

From 2011.igem.org

(Difference between revisions)
(Break It: Gluten Destruction Protocols)
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[https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction]
[https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction]
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[https://2011.igem.org/Team:Washington/alkanebiosynthesis_cloning Alkane Biosynthesis cloning]
[https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team]
[https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team]
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[https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Purified Enzyme Assay]
[https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Purified Enzyme Assay]
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=Make It: iGem Toolkits=
 
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=Make It: iGem Toolkits=
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]

Revision as of 21:32, 16 September 2011


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagensis

Overview of how Kunkel Mutagensis works

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks


Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Alkane Biosynthesis cloning

Cell Lysate Assay by Decarbonylase Redesign Team


Break It: Gluten Destruction Protocols

Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification

Purified Enzyme Assay


Make It: iGem Toolkits

Cytometry Protocol

Electroporation (Transformation)

Gibson Cloning/Assembly

Gibson Purification

High-Yield PCR (Full-Gene Assembly)

Isolation of Plasmid DNA (miniprep)

Induction Studies of Proteins Fusions (mam-sfGFP)

PBS Stock Protocol

Preparation of Overnight Cultures


Wiki Design

Wiki Design Tools (Wiki Markup, WikiDust, etc.)