Team:Washington/Protocols

From 2011.igem.org

(Difference between revisions)
(Make It: Diesel Production Protocols)
(Make It: Magnetosome Protocols)
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[https://2011.igem.org/Team:Washington/Protocols/Micros. Microscopy Protocol]
[https://2011.igem.org/Team:Washington/Protocols/Micros. Microscopy Protocol]
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[https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol]
[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]
[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]

Revision as of 05:13, 14 September 2011


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagensis

Overview of how Kunkel Mutagensis works

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks


Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Cell Lysate Assay by Decarbonylase Redesign Team

Break It: Gluten Destruction Protocols

Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification


Make It: Magnetosome Protocols

Cytometry Protocol

Electroporation (Transformation)

Gibson Cloning/Assembly

Gibson Purification

High-Yield PCR (Full-Gene Assembly)

Isolation of Plasmid DNA (miniprep)

Microscopy Protocol

PBS Stock Protocol

Preparation of Overnight Cultures

Wiki Design

Wiki Design Tools (Wiki Markup, WikiDust, etc.)