Team:Washington/Parts

From 2011.igem.org

(Difference between revisions)
(Improved Parts)
(Improved Parts)
Line 43: Line 43:
==''Improved Parts''==
==''Improved Parts''==
-
::'''8.''' [http://partsregistry.org/Part:BBa_K590059 BBa_K590059], [http://partsregistry.org/Part:BBa_K590060 BBa_K590060], [http://partsregistry.org/Part:BBa_K590061 BBa_K590061: '''LuxC, D, and E'''] (Cambridge, iGEM 2010) - Formerly parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K325902 BBa_K325902] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K325903 BBa_K325903].  ''luxC, D,'' and ''E'' were not separated or codon-optimized.  We codon-optimized these genes and put them under the control of standard Elowitz RBS's.  This was accomplished by the Alternate Aldehyde branch of the Alkane Production team.
+
::'''8.''' [http://partsregistry.org/Part:BBa_K590059 BBa_K590059], [http://partsregistry.org/Part:BBa_K590060 BBa_K590060], [http://partsregistry.org/Part:BBa_K590061 BBa_K590061: '''LuxC, D, and E'''] (Cambridge, iGEM 2010) - Formerly part of the [http://partsregistry.org/Part:BBa_K325909 LuxBrick] the genes ''luxC, D,'' and ''E'' were not separated or codon-optimized.  We codon-optimized these genes and put them under the control of standard Elowitz RBS's.  This was accomplished by the Alternate Aldehyde branch of the Alkane Production team.
='''All Submitted Parts'''=
='''All Submitted Parts'''=
<center><groupparts>iGEM011 Washington</groupparts></center>
<center><groupparts>iGEM011 Washington</groupparts></center>

Revision as of 07:38, 23 September 2011


Data Page




Data Summary

Data for Favorite New Parts

Diesel Production

1, 2. BBa_K590025: The PetroBrick - A modular and open platform for the biological production of diesel fuel. The PetroBrick consists of AAR and ADC, each behind a standard Elowitz RBS. All of this is under regulation by a high constitutive promoter in pSB1C3.

Gluten Destruction

3. BBa_K590023: KumamaMax- A modified version of the enzyme Kumamolisin, a protease ofthe sedolisin family native to Alicyclobacillus sendaiensis known to be active at low pH and elevated temperatures. To Kumamolisin, the mutations N291D, G319S D358G, D368H increase activity to the PQLP peptide, an antigenic epitope in gliadin, 118-fold.

Gibson Assembly Toolkit

4. BBa_K590010: pGA1A3_pLacGFP, BBa_K590011: pGA1C3_pLacGFP, BBa_K590012: pGA4C5_pLacGFP, BBa_K590013: pGA4A5_pLacGFP, BBa_K590014: pGA3K3_pLacGFP - These are plasmid backbones optimized for use in Gibson cloning, with a variety of copy numbers and antibiotic resistances.

Magnetosome Toolkit

5. BBa_K590015: sfGFP_mamK_pGA1C3 - This part consists of the mamK gene from Magnetospirillum magneticum strain AMB-1, fused to sfGFP, in the backbone of pGA1C3. MamK was previously reported to be required for proper alignment of magnetosomes in a chain in magnetic bacteria.
6. BBa_K590016 sfGFP_mamI_pGA1C3 - This part consists of mamI gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. MamI is a membrane-localized protein that localizes magnetic vesicles to the surface of cells, thus forming characteristic magnetosome chains.

Data for Existing Parts

7. K314100: High Constitutive Expression Cassette (Washington, iGEM 2010) - We used this part to express our Petrobrick.

Improved Parts

8. BBa_K590059, BBa_K590060, BBa_K590061: LuxC, D, and E (Cambridge, iGEM 2010) - Formerly part of the LuxBrick the genes luxC, D, and E were not separated or codon-optimized. We codon-optimized these genes and put them under the control of standard Elowitz RBS's. This was accomplished by the Alternate Aldehyde branch of the Alkane Production team.

All Submitted Parts

Loading.....