Team:Washington/Magnetosomes/Results

From 2011.igem.org

(Difference between revisions)
(What' in the toolkit and How We Assembled It)
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Our genes of interest were mamK and mamI as they have functions related to localization of the magnetosome. Specifically, mamK is a bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. mamK is also shown to localize the mamI, which is loss inhibits membrane formation.  
Our genes of interest were mamK and mamI as they have functions related to localization of the magnetosome. Specifically, mamK is a bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. mamK is also shown to localize the mamI, which is loss inhibits membrane formation.  
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For more information, please refer to the table below:
+
For more information, refer to the table below:
{| class="wikitable"
{| class="wikitable"
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| mamAB
| mamAB
| Specific
| Specific
 +
| Vesicle, (Chain Formation?)
 +
| >berkeley 2010: Loss causes no membrane formation, is localized onto chains
|-
|-
| mamE
| mamE
| amb0963
| amb0963
-
| mamAB
+
| mamAB; mam Islet
| Related
| Related
 +
|
 +
| >Membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
|-
|-
| mamJ
| mamJ
-
| 1538
+
| amb0964
 +
| mamAB; mam Islet
 +
| Specific
 +
| Chain Formation
 +
| >Proper magnetosome chain organization/assembly
|-
|-
-
| mamKL
+
| mamK
-
| 1336
+
| amb0965
 +
| mamAB; mam Islet
 +
| Related
 +
| Chain Formation
 +
| >required for proper magnetosome chain organization; *bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI
|-  
|-  
-
| mamMN
+
| mamL
-
| 2323  
+
| amb0965
 +
| mamAB; mam Islet
 +
| 2323
|-
|-
| mamO
| mamO

Revision as of 02:03, 19 September 2011


About the Magnetosome Toolkit:

Washington Methode image.jpg

Using standard synthetic biology protocols and the vectors we created in our Gibson Assembly Toolkit, our team was able to create a "Magnetosome Toolkit" consisting of the most basic parts required for magnetosome formation. Providing this toolkit allows future iGem teams to manipulate and understand magnetosome formation to one day create magnets in various types of bacteria.

What' in the toolkit and How We Assembled It

After piecing together the 16 kb genome of the mamAB gene cluster within the magnetosome island (MAI), we extracted out the genes in the following group:

Gene groups Length (bp)
mamHI 1541
mamE 2172
mamJ 1538
mamKL 1336
mamMN 2323
mamO 1914
mamPA 1493
mamQRB 2029
mamSTU 2030
mamV 1002
.

Using standard PCR protocols, these genes were extracted and..... These genes were visualized on gels and sequence confirmed; therefore, we can be sure that these are some of the many genes required for proper magnetosome formation.

As previously noted, magnetosome formation within the host-organism, Magnetospirillium magneticum, strain AMB-1, is a highly regulated step-wise process. As shown in (link: figure #), genes encode for an invagination in the inner membrane, there are genes which help align the magnetosomes into their characteristics chains, and there are genes which regulate the biomineralization of magnetic particles. Our team chose to focus on genes specifically related to magnetosome scaffolding/alignment, since it has many practical uses in synthetic biology (??? maybe we could make a link to the alkenes future if they wanted to use mamI....)


Our genes of interest were mamK and mamI as they have functions related to localization of the magnetosome. Specifically, mamK is a bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. mamK is also shown to localize the mamI, which is loss inhibits membrane formation. For more information, refer to the table below:

Gene AMB Number Cluster Membership Member of 28 genes list? (specific*/related**) Function Summary (Vesicle chain formation, and/or biomineralization) Gene Function
mamH amb0961 mamAB Related
mamI amb0962 mamAB Specific Vesicle, (Chain Formation?) >berkeley 2010: Loss causes no membrane formation, is localized onto chains
mamE amb0963 mamAB; mam Islet Related >Membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
mamJ amb0964 mamAB; mam Islet Specific Chain Formation >Proper magnetosome chain organization/assembly
mamK amb0965 mamAB; mam Islet Related Chain Formation >required for proper magnetosome chain organization; *bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI
mamL amb0965 mamAB; mam Islet 2323
mamO 1914
mamPA 1493
mamQRB 2029
mamSTU 2030
mamV 1002
.