Team:Washington/Magnetosomes/GibsonResults

From 2011.igem.org

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==Comparison between pGA and pSB vectors==
==Comparison between pGA and pSB vectors==
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To quantitatively illustrate that better cloning efficiency is achieved by using pGA vectors vs standard pSB vectors, a [https://2011.igem.org/Team:Washington/Protocols/pGA pGA vector assay] was conducted. The Gibson product made using our pGA vectors was plated over 6 plates.  
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To quantitatively illustrate that better cloning efficiency is achieved by using pGA vectors vs standard pSB vectors, a [https://2011.igem.org/Team:Washington/Protocols/pGA pGA vector assay] was conducted. The Gibson product made using our pGA vectors was plated over 6 plates, and the Gibson efficiency was calculated for each plate.  
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Efficiency was calculated by dividing the # of bright colonies by the # of total colonies - # of background colonies.
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Gibson Efficiency = Bright colonies/Total - Background)
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After calculating the Gibson efficiency for all 6 plates, the average efficiecy was calculated to be '''0.991129032'''.This is significantly higher than the efficiency calculated for the
   
   
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[[File:Washington IGEM2011 PLACEHOLDER PLATE1.JPG|500px]]  
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[[File:Washington IGEM2011 PLACEHOLDER PLATE1.JPG|500px]]
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(this is just a placeholder)
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Revision as of 20:34, 27 September 2011


Gibson Toolkits: Evaluation


Comparison between pGA and pSB vectors

To quantitatively illustrate that better cloning efficiency is achieved by using pGA vectors vs standard pSB vectors, a pGA vector assay was conducted. The Gibson product made using our pGA vectors was plated over 6 plates, and the Gibson efficiency was calculated for each plate.

Efficiency was calculated by dividing the # of bright colonies by the # of total colonies - # of background colonies. Gibson Efficiency = Bright colonies/Total - Background)



After calculating the Gibson efficiency for all 6 plates, the average efficiecy was calculated to be 0.991129032.This is significantly higher than the efficiency calculated for the


Washington IGEM2011 PLACEHOLDER PLATE1.JPG