Revision as of 01:18, 23 September 2011 by Mdsmith (Talk | contribs)

iGEM Toolkits: Background

As with the expansion of the iGEM competition, many iGEM teams have started to investigate the possibility of working with large-scale genomes. Large-scale gene manipulation often requires the use of tools which allow multiple gene inserts as to bring the cloning project from single gene level to a multiple gene level. However, the current BioBrick standard vectors available through iGEM are not designed for multiple-insert cloning. Therefore, the UW iGEM team decided to research methods to improve cloning efficiency and as a result, two "toolkits" were submitted to the registry.

Gibson Assembly Toolkit

As a continuation of the 2010 UW IGEM project, this year we developed and submitted several plasmid backbones that are Gibson cloning method friendly-- aka pGA vectors. It is called the Gibson Assembly Toolkit

Igem2011 GibsonToolkit.png
What's in the Gibson Assembly Toolkit?
  • Five plasmid backbones
  • 2 High copy extraction vectors: pGA1A3, pGA1C3
  • 3 low copy assembly vectors: pGA3K3, pGA4A5, pGA4C5

Magnetosome Toolkit

In addition, we were also ambitious about assembling a large gene-construct of over 16 kb. Therefore, utilizing our pGA vectors and Gibson cloning methods, the Magnetosome Toolkit was developed with the goal to build magnetic E.Coli; a novel characteristic expressed solely by magnetotactic bacteria, such as Magnetospirillum magneticum strain AMB-1.

Igem2011 MagnetToolkit.png

What’s in the Magnetosome Toolkit?

  • A set of the 18 essential genes for the various steps of magnetosome formation.
  • Our favorite genes in pGA vectors
  • A table compiling individual gene functions from our literature search