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Team:Washington/Magnetosomes/Background
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=== Gibson Assembly Toolkit === | === Gibson Assembly Toolkit === | ||
| - | As | + | As an expansion to work started by the [https://2010.igem.org/Team:Washington/Tools_Used/Next-Gen_Cloning 2010 UW IGEM project], this year we developed and submitted a set of plasmid backbones for cloning and expression of BioBricks that are optimized for Gibson assembly. We call these pGA vectors - they comprise the [https://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly Toolkit] |
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=== Magnetosome Toolkit === | === Magnetosome Toolkit === | ||
In addition, we were also ambitious about assembling a large gene-construct of over 16 kb. Therefore, utilizing our pGA vectors and Gibson cloning methods, the [https://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit] was developed with the goal to build magnetic ''E.Coli''; a novel characteristic expressed solely by magnetotactic bacteria, such as ''Magnetospirillum magneticum'' strain AMB-1. | In addition, we were also ambitious about assembling a large gene-construct of over 16 kb. Therefore, utilizing our pGA vectors and Gibson cloning methods, the [https://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit] was developed with the goal to build magnetic ''E.Coli''; a novel characteristic expressed solely by magnetotactic bacteria, such as ''Magnetospirillum magneticum'' strain AMB-1. | ||
Revision as of 01:45, 23 September 2011
As with the expansion of the iGEM competition, many iGEM teams have started to investigate the possibility of working with large-scale genomes. Large-scale gene manipulation often requires the use of tools which allow multiple gene inserts as to bring the cloning project from single gene level to a multiple gene level. However, the current BioBrick standard vectors available through iGEM are not designed for multiple-insert cloning. Therefore, the UW iGEM team decided to research methods to improve cloning efficiency and as a result, two "toolkits" were submitted to the registry.
Gibson Assembly Toolkit
As an expansion to work started by the 2010 UW IGEM project, this year we developed and submitted a set of plasmid backbones for cloning and expression of BioBricks that are optimized for Gibson assembly. We call these pGA vectors - they comprise the Gibson Assembly Toolkit
- What's in the Gibson Assembly Toolkit?
- Five plasmid backbones
- 2 High copy extraction vectors: pGA1A3, pGA1C3
- 3 low copy assembly vectors: pGA3K3, pGA4A5, pGA4C5
Magnetosome Toolkit
In addition, we were also ambitious about assembling a large gene-construct of over 16 kb. Therefore, utilizing our pGA vectors and Gibson cloning methods, the Magnetosome Toolkit was developed with the goal to build magnetic E.Coli; a novel characteristic expressed solely by magnetotactic bacteria, such as Magnetospirillum magneticum strain AMB-1.
What’s in the Magnetosome Toolkit?
- A set of the 18 essential genes for the various steps of magnetosome formation.
- Our favorite genes in pGA vectors
- A table compiling individual gene functions from our literature search
