Team:Washington/Celiacs/Results

From 2011.igem.org

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[[File:Washington Best Mutants.png|left|500px|thumb|Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.]]
[[File:Washington Best Mutants.png|left|500px|thumb|Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.]]
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[[File:Washington InitialKumavSC.png|left|500px|thumb|Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.]]

Revision as of 00:51, 16 September 2011



Over 100 unique mutants were screened with a whole cell lysate assay for improved activity on the PQLP model substrate.


Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.
Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.
Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.