Team:Washington/Celiacs/Results

From 2011.igem.org

(Difference between revisions)
Line 2: Line 2:
__NOTOC__
__NOTOC__
 +
==Testing our designed mutants for activity on PQLP==

Revision as of 01:18, 16 September 2011


Testing our designed mutants for activity on PQLP

Over 100 unique mutants were screened with a whole cell lysate assay for improved activity on the PQLP model substrate.


Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.
Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.
Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.