Team:Washington/Celiacs/Parts

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Gluten Destruction submitted four parts to the registry: Wild-type Kumamolisin-As and three of our promising mutants. The a short description about each part is provided below. Full documentation can be found on the Parts Registry.

BBa_K590021: Kumamolisin-As

An enzyme from the sedolisin family native to Alicyclobacillus sendaiensis known to be a collgenase active at low pH and elevated temperatures.

BBa_K590022: Kumamolisin-As_G319S, D358G, D368H

A mutated Kumamolisin-As enzyme aimed to combat celiac disease by increased specificity for the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residues 319, 358, and 368 from Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.

BBa_K590023: Kumamolisin-As_N291D

A mutated Kumamolisin-As enzyme aimed to combat celiac disease by increased specificity for the PQLP peptide, an antigenic epitope in gliadin. This mutant has a point mutation at residue 291 from Asparagine to Aspartate.

BBa_K590024: Kumamolisin-As_S354N, D358G, D368H]

A mutated Kumamolisin-As enzyme aimed to combat celiac disease by increased specificity for the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residue 354, 358, and 368 from Serine to Asparagine, Aspartate to Glycine, and Aspartate to Histidine, respectively.