Team:Washington/Celiacs/Parts

From 2011.igem.org

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Gluten Destruction submitted four parts to the registry: Wild-type Kumamolisin-As and three of our promising mutants. The a short description about each part is provided below. Full documentation can be found on the Parts Registry.
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<center><big><big><big><big>'''Gluten Destruction: Parts Submitted'''</big></big></big></big></center><br><br>
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590021 BBa_K590021]: Kumamolisin-As
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Gluten Destruction submitted five parts to the registry: Wild-type Kumamolisin-As and four of our promising mutants. A short description for each part is provided below.
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An enzyme from the sedolisin family native to Alicyclobacillus sendaiensis known to be a collgenase active at low pH and elevated temperatures.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590021 BBa_K590021: '''Kumamolisin-As''']
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590022 BBa_K590022]: Kumamolisin-As_G319S, D358G, D368H
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An enzyme from the sedolisin family native to ''Alicyclobacillus sendaiensis'' with known collagenase activity at low pH and elevated temperatures.
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A mutated Kumamolisin-As enzyme aimed to combat celiac disease by increased specificity for the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residues 319, 358, and 368 from Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590022 BBa_K590022: '''Kumamolisin-As_G319S, D358G, D368H''']
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590023 BBa_K590023]: Kumamolisin-As_N291D
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A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residues 319, 358, and 368 from Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.
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A mutated Kumamolisin-As enzyme aimed to combat celiac disease by increased specificity for the PQLP peptide, an antigenic epitope in gliadin. This mutant has a point mutation at residue 291 from Asparagine to Aspartate.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590023 BBa_K590023: '''Kumamolisin-As_N291D''']
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590024 BBa_K590024]: Kumamolisin-As_S354N, D358G, D368H]
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A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant has a point mutation at residue 291 from Asparagine to Aspartate.
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A mutated Kumamolisin-As enzyme aimed to combat celiac disease by increased specificity for the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residue 354, 358, and 368 from Serine to Asparagine, Aspartate to Glycine, and Aspartate to Histidine, respectively.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590024 BBa_K590024: '''Kumamolisin-As_S354N, D358G, D368H''']
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A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residue 354, 358, and 368 from Serine to Asparagine, Aspartate to Glycine, and Aspartate to Histidine, respectively.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590087 BBa_K590087: '''KumaMax (aka, Kumamolisin-As_N291D, G319S, D358G, D368H)''']
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A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.

Latest revision as of 06:42, 13 October 2011


Gluten Destruction: Parts Submitted


Gluten Destruction submitted five parts to the registry: Wild-type Kumamolisin-As and four of our promising mutants. A short description for each part is provided below.

BBa_K590021: Kumamolisin-As

An enzyme from the sedolisin family native to Alicyclobacillus sendaiensis with known collagenase activity at low pH and elevated temperatures.

BBa_K590022: Kumamolisin-As_G319S, D358G, D368H

A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residues 319, 358, and 368 from Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.

BBa_K590023: Kumamolisin-As_N291D

A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant has a point mutation at residue 291 from Asparagine to Aspartate.

BBa_K590024: Kumamolisin-As_S354N, D358G, D368H

A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant has point mutations at residue 354, 358, and 368 from Serine to Asparagine, Aspartate to Glycine, and Aspartate to Histidine, respectively.

BBa_K590087: KumaMax (aka, Kumamolisin-As_N291D, G319S, D358G, D368H)

A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity on the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.