Team:Washington/Alkanes/Results
From 2011.igem.org
GC-MS analysis shows that our alkane production system is working
We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected.
No significant hydrocarbons peaks in samples extracted from cells expressing only ADC
No significant peaks are found on the ADC-Only control GC plot within the time range that we expect alkane signal based upon GC-MS runs on standards(8-10.5 minutes). This is expected, as E. coli does not normally produce any long chain length aldehyde substrates.