Team:Washington/Alkanes/Results

From 2011.igem.org

GC-MS analysis shows that our alkane production system is working

We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected.

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

No significant hydrocarbons produced by cells expressing only ADC

No significant peaks are found on the ADC-Only control GC plot within the time range that we expect alkane signal based upon GC-MS runs on standards(8-10 minutes). This is expected, as E. coli does not normally produce any long chain length aldehyde substrates. ===C16-Aldehyde production