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Team:Washington/Alkanes/Results
From 2011.igem.org
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We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected. | We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected. | ||
[[File:Washinton_AARADCGC-MS.png|center|800px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]] | [[File:Washinton_AARADCGC-MS.png|center|800px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]] | ||
| - | ===No significant hydrocarbons | + | ===No significant hydrocarbons peaks in samples extracted from cells expressing only ADC=== |
| - | No significant peaks are found on the ADC-Only control GC plot within the time range that we expect alkane signal based upon GC-MS runs on standards(8-10 minutes). This is expected, as ''E. coli'' does not normally produce any long chain length aldehyde substrates. | + | No significant peaks are found on the ADC-Only control GC plot within the time range that we expect alkane signal based upon GC-MS runs on standards(8-10.5 minutes). This is expected, as ''E. coli'' does not normally produce any long chain length aldehyde substrates. |
| - | ===C16- | + | ===C16-Alcohol production by cells expressing AAR=== |
Revision as of 21:53, 11 September 2011
GC-MS analysis shows that our alkane production system is working
We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected.
No significant hydrocarbons peaks in samples extracted from cells expressing only ADC
No significant peaks are found on the ADC-Only control GC plot within the time range that we expect alkane signal based upon GC-MS runs on standards(8-10.5 minutes). This is expected, as E. coli does not normally produce any long chain length aldehyde substrates.
