Team:Washington/Alkanes/Results
From 2011.igem.org
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+ | ===GC-MS analysis shows that our alkane production system is working=== | ||
We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected. | We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected. | ||
- | [[File:Washinton_AARADCGC-MS.png|center|800px|thumb|GC | + | [[File:Washinton_AARADCGC-MS.png|center|800px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]] |
+ | ===No significant hydrocarbons produced by cells expressing | ||
+ | No significant peaks are found on the ADC-Only control GC plot. This is expected, as ''E. coli'' does not normally produce any long chain length substrates |
Revision as of 21:28, 11 September 2011
GC-MS analysis shows that our alkane production system is working
We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected.
===No significant hydrocarbons produced by cells expressing No significant peaks are found on the ADC-Only control GC plot. This is expected, as E. coli does not normally produce any long chain length substrates