Team:Washington/Alkanes/Results

From 2011.igem.org

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===GC-MS analysis shows that our alkane production system is working===
We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected.
We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected.
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[[File:Washinton_AARADCGC-MS.png|center|800px|thumb|GC-MS traces showing that alkanes are only produced when both ADC and AAR are expressed.]]
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[[File:Washinton_AARADCGC-MS.png|center|800px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
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===No significant hydrocarbons produced by cells expressing
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No significant peaks are found on the ADC-Only control GC plot. This is expected, as ''E. coli'' does not normally produce any  long chain length substrates

Revision as of 21:28, 11 September 2011

GC-MS analysis shows that our alkane production system is working

We proformed GC-MS analysis on cell cultures expressing both Acyl-ACP reductase(AAR) and Aldehyde Decarbonylase(ADC). to act as controls and in order to show that the alkane production system is working as expected.

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

===No significant hydrocarbons produced by cells expressing No significant peaks are found on the ADC-Only control GC plot. This is expected, as E. coli does not normally produce any long chain length substrates