Team:Washington/Alkanes/Parts

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Diesel Production: Parts Submitted


The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the Parts Registry.

Composite

BBa_K590025 "The Petrobrick"

A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of AAR and ADC, each behind a standard Elowitz RBS. All of this in psb1C3 with a high constitutive promoter.

BBa_K590026 ADC-PSB1C3-High constitutive

The aldehyde decarbonylase from The Petrobrick behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.

BBa_K590027 AAR-PSB1C3-High consititutive

The acyl-ACP reductase from The Petrobrick behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.

Coding

BBa_K590031 Synechococcus elongatus PCC7942 Aldehyde Decarbonylase

This part encodes an enzyme (Aldehyde Decarbonylase (ADC)) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate.

BBa_K590032 Acyl-ACP Reductase

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for E. coli.