Team:Washington/Alkanes/Parts

From 2011.igem.org

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The diesel production sub-team submitted many parts, but the below are our favorites along with a short description.  The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry].  
The diesel production sub-team submitted many parts, but the below are our favorites along with a short description.  The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry].  
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=='''Composite'''==
=='''Composite'''==
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The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
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=='''Coding'''==
=='''Coding'''==

Revision as of 03:45, 28 September 2011


Diesel Production: Parts Submitted


The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the Parts Registry.


Composite

BBa_K590025 "The Petrobrick"

A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of AAR and ADC, each behind a standard Elowitz RBS. All of this in psb1C3 regulated by a high constitutive promoter.

BBa_K590026 ADC-PSB1C3-High constitutive

The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.

BBa_K590027 AAR-PSB1C3-High consititutive

The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.


Coding

BBa_K590031 Synechococcus elongatus PCC7942 Aldehyde Decarbonylase

This part encodes an enzyme (Aldehyde Decarbonylase (ADC)) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. This part has been codon-optimized for E. coli.

BBa_K590032 Acyl-ACP Reductase

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for E. coli.