Team:Washington/Alkanes/Parts

From 2011.igem.org

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<center><big><big><big><big>Diesel Production: Parts Submitted</big></big></big></big></center><br><br>
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<center><big><big><big><big>'''Diesel Production: Parts Submitted'''</big></big></big></big></center><br><br>
The diesel production sub-team submitted many parts, but the below are our favorites along with a short description.  The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry].  
The diesel production sub-team submitted many parts, but the below are our favorites along with a short description.  The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry].  
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=='''Composite'''==
[http://partsregistry.org/Part:BBa_K590025 BBa_K590025 '''"The Petrobrick"''']
[http://partsregistry.org/Part:BBa_K590025 BBa_K590025 '''"The Petrobrick"''']
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A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of a high constitutive promoter, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 AAR], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 ADC], each with a standard Elowitz RBS. All of this in psb1C3 along with the f1 origin that necessary in Kunkel mutagenesis.
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A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 AAR] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 ADC], each behind a standard Elowitz RBS. All of this in psb1C3 regulated by a high constitutive promoter.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590026 BBa_K590026 '''ADC-PSB1C3-High constitutive''']
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590026 BBa_K590026 '''ADC-PSB1C3-High constitutive''']
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The aldehyde decarbonylase from The Petrobrick behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
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The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590027 BBa_K590027 '''AAR-PSB1C3-High consititutive''']
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590027 BBa_K590027 '''AAR-PSB1C3-High consititutive''']
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The acyl-ACP reductase from The Petrobrick behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
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The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
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=='''Coding'''==
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 BBa_K590031 '''Synechococcus elongatus PCC7942 Aldehyde Decarbonylase''']
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This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate.  This part has been codon-optimized for ''E. coli''.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 BBa_K590032 '''Acyl-ACP Reductase''']
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This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for ''E. coli''.

Latest revision as of 03:45, 28 September 2011


Diesel Production: Parts Submitted


The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the Parts Registry.


Composite

BBa_K590025 "The Petrobrick"

A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of AAR and ADC, each behind a standard Elowitz RBS. All of this in psb1C3 regulated by a high constitutive promoter.

BBa_K590026 ADC-PSB1C3-High constitutive

The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.

BBa_K590027 AAR-PSB1C3-High consititutive

The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.


Coding

BBa_K590031 Synechococcus elongatus PCC7942 Aldehyde Decarbonylase

This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. This part has been codon-optimized for E. coli.

BBa_K590032 Acyl-ACP Reductase

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for E. coli.