Team:Washington/Alkanes/Parts

From 2011.igem.org

(Difference between revisions)
(Make It: Parts Submitted)
(Coding)
 
(23 intermediate revisions not shown)
Line 2: Line 2:
__NOTOC__
__NOTOC__
-
=Make It: Parts Submitted=
+
<center><big><big><big><big>'''Diesel Production: Parts Submitted'''</big></big></big></big></center><br><br>
-
The Petrobrick
+
The diesel production sub-team submitted many parts, but the below are our favorites along with a short description.  The comprehensive list can be found on the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Washington Parts Registry].
-
<html>
+
 
-
<img src=http://2011.igem.org/wiki/images/3/3d/Washington_wikidust_petrobrick.png width="600" height="282" usemap="#washington_wikidust_petrobrick.png"/>
+
=='''Composite'''==
-
<map name="washington_wikidust_petrobrick.png">
+
 
-
<area shape="rect" coords="435,59,601,224" title="Part:BBa_C0024" href="http://partsregistry.org/Part:BBa_C0024"/>
+
[http://partsregistry.org/Part:BBa_K590025 BBa_K590025 '''"The Petrobrick"''']
-
<area shape="rect" coords="187,59,353,224" title="Part:BBa_K106001" href="http://partsregistry.org/Part:BBa_K106001"/>
+
 
-
<area shape="rect" coords="105,100,187,183" title="Part:BBa_B0034" href="http://partsregistry.org/Part:BBa_B0034"/>
+
A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 AAR] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 ADC], each behind a standard Elowitz RBS. All of this in psb1C3 regulated by a high constitutive promoter.
-
<area shape="rect" coords="353,100,435,183" title="Part:BBa_B0034" href="http://partsregistry.org/Part:BBa_B0034"/>
+
 
-
<area shape="rect" coords="2,68,105,171" title="Part:BBa_J23100" href="http://partsregistry.org/Part:BBa_J23100"/>
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590026 BBa_K590026 '''ADC-PSB1C3-High constitutive''']
-
</map>
+
 
-
</html>
+
The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
 +
 
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590027 BBa_K590027 '''AAR-PSB1C3-High consititutive''']
 +
 
 +
The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.
 +
 
 +
 
 +
=='''Coding'''==
 +
 
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 BBa_K590031 '''Synechococcus elongatus PCC7942 Aldehyde Decarbonylase''']
 +
 
 +
This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate.  This part has been codon-optimized for ''E. coli''.
 +
 
 +
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 BBa_K590032 '''Acyl-ACP Reductase''']
 +
 
 +
This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for ''E. coli''.

Latest revision as of 03:45, 28 September 2011


Diesel Production: Parts Submitted


The diesel production sub-team submitted many parts, but the below are our favorites along with a short description. The comprehensive list can be found on the Parts Registry.


Composite

BBa_K590025 "The Petrobrick"

A modular and open platform for the biological production of diesel fuel. The Petrobrick consists of AAR and ADC, each behind a standard Elowitz RBS. All of this in psb1C3 regulated by a high constitutive promoter.

BBa_K590026 ADC-PSB1C3-High constitutive

The aldehyde decarbonylase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.

BBa_K590027 AAR-PSB1C3-High consititutive

The acyl-ACP reductase behind a high constitutive promoter and an Elowitz RBS. This was an important negative control when testing for alkane production.


Coding

BBa_K590031 Synechococcus elongatus PCC7942 Aldehyde Decarbonylase

This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. This part has been codon-optimized for E. coli.

BBa_K590032 Acyl-ACP Reductase

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. This part has been codon optimized for E. coli.