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Team:Washington/Alkanes/Future/Vector
From 2011.igem.org
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*C16 Alcohol Observed | *C16 Alcohol Observed | ||
| + | ==Effect of Trace metals on yield== | ||
| + | We had suspected that the trace metals in the original media may be inhibiting alkane production. We analzed alkane production of cells transformed with the basic Petrobrick in medias of varying trace metal concentration. | ||
Glass vs Plastic | Glass vs Plastic | ||
Revision as of 04:55, 15 September 2011
Using our initial, non-optimized growth conditions, we were able to obtain alkane/ene yields of approximently 2 mg/L. However,(reference) reported that they were able to obtain yields of as high as 300 mg/L, though growth and media conditions for obtaining this high yield was not given in either the paper, or supplementary materials. Therefore, we thought that by varying media and growth conditions, we would be able to dramatically increase yield. This page summerizes our optimization tests, and shows how we were able to improve yields to over 100mg/L.
Growth Optimization
- Total Alkane Production
- C16 Alcohol Observed
Effect of Trace metals on yield
We had suspected that the trace metals in the original media may be inhibiting alkane production. We analzed alkane production of cells transformed with the basic Petrobrick in medias of varying trace metal concentration. Glass vs Plastic
MG vs XL
Covered vs Open
Aerobic vs Microaerobic
1C3 vs 3D4R
TB vs M9
Trace Metals Titration
and more...
