Team:Washington/Alkanes/Future/Vector

From 2011.igem.org

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Using our initial, non-optimized growth conditions, we were able to obtain alkane/ene yields of approximently 2 mg/L. However,(reference) reported that they were able to obtain yields of as high as 300 mg/L, though growth and media conditions for obtaining this high yield was not given in either the paper, or supplementary materials. Therefore, we thought that by varying media and growth  conditions, we would be able to dramatically increase yield. This page summerizes our optimization tests, and shows how we were able to improve yields to over 100mg/L.
'''Growth Optimization'''
'''Growth Optimization'''
*Total Alkane Production
*Total Alkane Production
*C16 Alcohol Observed
*C16 Alcohol Observed
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Glass vs Plastic
Glass vs Plastic

Revision as of 21:38, 14 September 2011

Using our initial, non-optimized growth conditions, we were able to obtain alkane/ene yields of approximently 2 mg/L. However,(reference) reported that they were able to obtain yields of as high as 300 mg/L, though growth and media conditions for obtaining this high yield was not given in either the paper, or supplementary materials. Therefore, we thought that by varying media and growth conditions, we would be able to dramatically increase yield. This page summerizes our optimization tests, and shows how we were able to improve yields to over 100mg/L. Growth Optimization

  • Total Alkane Production
  • C16 Alcohol Observed

Glass vs Plastic

MG vs XL

Covered vs Open

Aerobic vs Microaerobic

1C3 vs 3D4R

TB vs M9

Trace Metals Titration

and more...