Team:Washington

From 2011.igem.org

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<center><big><big><big><big>'''Make It or Break It: <br/><br/> Diesel Production and Gluten Destruction, <br/><br/>the Synthetic Biology Way'''</big></big></big></big></center><br>
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=<center>'''Make It or Break It: <br/> Diesel Production and Gluten Destruction, the Synthetic Biology Way'''</center>=
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Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.
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<center>Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.</center>
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[[Image:Washington_Fire.jpg|left|320px|borderless|link=https://2011.igem.org/Team:Washington/Alkanes/Background]]
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[[Image:Washington_Bottle.jpg|right|200px|borderless]]
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[[Image:Washington_Bottle.jpg|right|200px|borderless|link=https://2011.igem.org/Team:Washington/Celiacs/Background]]
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[https://2011.igem.org/Team:Washington/Alkanes/Background '''Make It: Diesel Production''']  We constructed a strain of ''Escherichia coli'' that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown convert fatty acid synthesis intermediates into alkanes.   
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[https://2011.igem.org/Team:Washington/Alkanes/Background '''Make It: Diesel Production''']  We constructed a strain of ''Escherichia coli'' that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown to convert fatty acid synthesis intermediates into alkanes.   
[https://2011.igem.org/Team:Washington/Celiacs/Background '''Break It: Gluten Destruction''']  We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.   
[https://2011.igem.org/Team:Washington/Celiacs/Background '''Break It: Gluten Destruction''']  We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.   
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[https://2011.igem.org/Team:Washington/Magnetosomes/Background '''iGEM Toolkits''']  To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to bring to the Parts Registry the Magnetosome Toolkit: a set of 18 genes from an essential operon in magnetotactic bacteria which we are characterizing to create magnetic ''E. coli''.
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[https://2011.igem.org/Team:Washington/Magnetosomes/Background '''iGEM Toolkits''']  To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to create the Magnetosome Toolkit: a set of 18 genes from an essential operon in magnetotactic bacteria which we are characterizing to create magnetic ''E. coli''.
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[[File:Washington_OSLI.png|frameless|border|link=http://www.osli.ca|Oil Sands Leadership Intiative]]
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[[File:Washington_UniversitySeal.gif|frameless|border|110px|link=http://http://www.washington.edu|University of Washington]]
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[[File:Washington_UniversitySeal.gif|frameless|border|100px|link=http://www.washington.edu|University of Washington]]
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[[File:Washington_Anaspec.gif|frameless|border|120px|link=http://www.anaspec.com|Anaspec]]
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[[File:Washington_ARPA-E_Logo.png|frameless|border|150px|link=http://arpa-e.energy.gov/ProgramsProjects/Electrofuels.aspx|Advanced Research Projects Agency - Energy]]
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[[File:Washington_ARPA-E_Logo.png|frameless|border|link=http://arpa-e.energy.gov/ProgramsProjects/Electrofuels.aspx|Advanced Research Projects Agency - Energy]]
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[[File:Washington2011_Hhmi_362_72.jpg|link=http://www.hhmi.org/|Howard Hughes Medical Institute]]
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[[File:Washington2011_NSFlogo.jpg|frameless|border|link=http://www.nsf.gov/|National Science Foundation]]
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[[File:Washington_Anaspec.gif|frameless|border|100px|link=http://www.anaspec.com|Anaspec]]

Latest revision as of 17:01, 2 December 2011


Make It or Break It:
Diesel Production and Gluten Destruction, the Synthetic Biology Way

Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.


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borderless

Make It: Diesel Production We constructed a strain of Escherichia coli that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown to convert fatty acid synthesis intermediates into alkanes.

Break It: Gluten Destruction We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.

iGEM Toolkits To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to create the Magnetosome Toolkit: a set of 18 genes from an essential operon in magnetotactic bacteria which we are characterizing to create magnetic E. coli.


Washington Spacer.jpg UW Diesel Front Page.png Washington Spacer.jpg UW Toolkits Front Page.png Washington Spacer.jpg UW Gluten Front Page.png Washington Spacer.jpg



Washington Spacer.jpg Oil Sands Leadership Intiative Washington Spacer.jpg University of Washington Washington Spacer.jpg Advanced Research Projects Agency - Energy Washington Spacer.jpg Howard Hughes Medical Institute Washington Spacer.jpg National Science Foundation Washington Spacer.jpg Anaspec