Team:Warsaw/ExpressionAdaptors

From 2011.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 11: Line 11:
-
<h2>Project description</h2>
+
<h2>We had a dream about fully standardized parts</h2>
<!-- <div class="note">Here goes something about our project</div> -->
<!-- <div class="note">Here goes something about our project</div> -->
Line 21: Line 21:
<div align="justify">
<div align="justify">
So far all the RBS parts were measured with GFP. We assumed that the strength of RBS is standard – does not depend on the expressed protein. But it turns out that the world is not as perfect as we would like it to be: it was during the 2010 iGEM competition that we came across the first sings that something is wrong. To investigate this problem further, this year we measured several RBS parts with various fluorescent proteins. Unfortunately, we were right.
So far all the RBS parts were measured with GFP. We assumed that the strength of RBS is standard – does not depend on the expressed protein. But it turns out that the world is not as perfect as we would like it to be: it was during the 2010 iGEM competition that we came across the first sings that something is wrong. To investigate this problem further, this year we measured several RBS parts with various fluorescent proteins. Unfortunately, we were right.
-
</div>
+
</div><br />
<div class="note">The Problem</div>
<div class="note">The Problem</div>
-
Variances in strength of RBS parts are caused by different mRNA fold. The beginning of every protein influences mRNA fold, which in turn makes the RBS sequence more or less accessible for the ribosome. If the RBS becomes a part of a secondary structure, ribosome is less likely to bind to it and the protein expression is limited or even impossible. </div>
+
<div align="justify">
 +
Variances in strength of RBS parts are caused by different mRNA fold. The beginning of every protein influences mRNA fold, which in turn makes the RBS sequence more or less accessible for the ribosome. If the RBS becomes a part of a secondary structure, ribosome is less likely to bind to it and the protein expression is limited or even impossible.  
 +
</div>
<br>
<br>

Latest revision as of 00:15, 21 September 2011

Example Tabs

We had a dream about fully standardized parts


Introduction
So far all the RBS parts were measured with GFP. We assumed that the strength of RBS is standard – does not depend on the expressed protein. But it turns out that the world is not as perfect as we would like it to be: it was during the 2010 iGEM competition that we came across the first sings that something is wrong. To investigate this problem further, this year we measured several RBS parts with various fluorescent proteins. Unfortunately, we were right.

The Problem
Variances in strength of RBS parts are caused by different mRNA fold. The beginning of every protein influences mRNA fold, which in turn makes the RBS sequence more or less accessible for the ribosome. If the RBS becomes a part of a secondary structure, ribosome is less likely to bind to it and the protein expression is limited or even impossible.